Biosolution R&D Center
Cell therapy products of Biosolution Co., Ltd. are divided into two groups, one including products for skin diseases, and the second including products for articular cartilage diseases. KeraHeal, KeraHeal-Allo and PlusubGel are developed as skin therapeutics for burns and chronic incurable ulcers, while KeraHeal-II is developed for treatment of hypo-/hyper-pigmented disorders. Also, we developed several osteoarthritis therapeutics such as CartiLife, CartiLife-Allo and LipoCartigen for treatment of articular cartilage lesions caused by osteoarthritis or trauma.
What is Skin?
Skin, the largest organ of the body, plays a key role in protecting the body against pathogens and excessive water loss. It is comprised of the epidermis, dermis, and the subcutaneous layer underneath. More than 90% of the epidermis consists of keratinocytes, and the remaining area contains melanocytes, Langerhans cells etc.
The avascular epidermal layer, comprised mainly of keratinocytes, provides the barrier function of the skin. The epidermis is composed of 4 layers called the stratum corneum, granular cell layer, spinous cell layer and the basal cell layer. Epidermal stem cells are found in the basal cell layer and are responsible for the daily regeneration of the layers in the epidermis.
Different Types of Skin Diseases
Skin can be damaged by different types of skin diseases as shown below.
Burns are a type of injury caused on the skin or other tissues due to heat,
electricity, chemicals, friction, or radiation.
|Type||Tissue Damage||Appearance||Symptoms||Time to Heal||Scars|
|1st Degree Burns||Epidermis||Rubefaction, Hyperemia||Pain, Hot Flushes||Several days||None|
|2nd Degree Burns
(Superficial, Epithelial Layer)
|Severe Pain, Hot Flushes,
Dullness in Sensibility
|About 2 weeks||In Some Cases|
|2nd Degree Burns
|Deeper Dermis||Blisters, Rubefaction,
Swelling, Wet, Blanched
|Less Pain, Noticable
Dullness in Sensitivity
|About 3 weeks||Easily Scarred|
|3rd Degree Burns||Entire Dermis,
|Painless, Loss in
|Painless, Loss in
does not Regenerate
The fundamental goal of burn treatment is to prevent infections and regenerate the epithelial layers of the wounds at the earliest opportunity. Primarily used as therapeutics for burn treatment, skin substitutes were developed as an alternative to skin grafts, especially for burn patients; however, it is difficult to cover a large area of damaged skin using skin substitutes.
Intractable ulcers are conditions caused by ulcerative damage on the epidermal and dermal region of the skin and occur in areas with insufficient blood flow. They are congenital or acquired through irritants, injury, and problems with auto-immunity and include conditions such as diabetic foot ulcers and pressure ulcers.
[Diabetic foot ulcer]
Intractable ulcers such as diabetic foot ulcers and pressure ulcers even result in hand or foot amputation and create profound psychological stress while interfering with daily lives. Therefore, there is a strong need for the development of new therapeutics.
Vitiligo is a chronic skin condition characterized by white spots forming on the skin due to a lack of melanin pigments. It presumedly occurs due to genetic, immune, and self-destructive melanocytes.
Along with vitiligo, achromic nevus, pityriasis alba and hypochromic scars trigger severe psychological stress and interfere with daily activities; development of new therapeutics is urgent.
Development of the Next-Generation Therapeutics for Skin Conditions
Biosolution Co., Ltd. is developing safe and effective skin therapeutics such as highly concentrated skin stem cells, complex skin cells including melanic pigments, and wound healing peptides in the body that take part in stem cell mobilization.
Articular Cartilage and Osteoarthritis
As we age, the articular cartilage slowly wears out and progresses into degenerative arthritis. The self-regenerative capabilities of the cartilage are extremely limited; therefore a lack of treatment on damaged cartilage can lead to osteoarthritis, which in turn can cause a decrease in the quality of life due to pain and difficulty in movement.
Current Treatment Options for Damaged Articular Cartilage
|Microfracture/Drilling||Osteochondral Graft/ Mosaicplasty||Total Arthroplasty|
A surgical method that drills small holes down to the subchondral bone for the stem cells and blood to be introduced from the bone marrow.
A method that transplants normal cartilage (cartilage that does not take weight load) or allogeneic cartilage into the lesion.
A method that replaces the joint surface with a metallic artificial joint. It limits the range of movement in the joint after surgery and can induce osteolysis to its neighboring bone tissue.
1), 2), 4): “Overview of Treatment Options for Articular Cartilage Repair: Past Present & Future” (May 15, 2009 by Ken Zaslav, M.D.)M
3): J Bone Joint Surg Am. 2004;86:65-72.
Cell Therapeutics, the Next-Generation Biopharmaceuticals
To improve on the weaknesses of existing therapeutics, companies are developing cell therapeutics, the next-gen biopharmaceuticals using cell culture technology. However, a product that is able to regenerate cartilage that can carry out the functions of the articular cartilage and have the ability to regenerate hyaline cartilage in an arthritis environment is desperate both in and out of the country.
Biosolution Co., Ltd. does more than the 1st-gen cell technology that simply proliferates the cells; we use a next-gen cell application technology to develop a small spherical cartilage called CartiLife, which uses scaffold-free tissue engineering technology to regenerate articular cartilage, and to develop LipoCartigen which is made through the transfection of genes that promote cartilage differentiation.
Alternatives to Animal Testing
Alternatives to Animal Testing are new or amended test methods that have a basis on the 3R rule which stands for the Refinement by minimizing the pain inflicted on experimental animals, Reduction of the number of experimental animals, and the Replacement by using lower forms of life or cells.
Using human tissue models as alternatives to animal testing will improve on the errors within previous animal test results to predict a more accurate human response and is under vigorous research as an alternative due to the prohibition of animal testing.
Animal testing on cosmetics is prohibited in the EU, thereby advising alternative test methods. This movement of prohibiting animal testing is expanding over to medicine, medical devices, chemical substances etc.
The Cosmetics Act in Korea has also been amended to include restrictions on animal testing in the year 2015.